Ultrafast microwave energy fixation for electron microscopy.
نویسندگان
چکیده
منابع مشابه
Scanning ultrafast electron microscopy.
Progress has been made in the development of four-dimensional ultrafast electron microscopy, which enables space-time imaging of structural dynamics in the condensed phase. In ultrafast electron microscopy, the electrons are accelerated, typically to 200 keV, and the microscope operates in the transmission mode. Here, we report the development of scanning ultrafast electron microscopy using a f...
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We investigated microwave-stimulated fixation of tissues for transmission electron microscopy using a domestic microwave oven operating at a frequency of 2.45 GHz with an output power of 500W. Microwave-stimulated fixation, in 4% glutaraldehyde, of fresh rat kidney, liver, heart and brain tissues was compared to conventional fixation. Human renal biopsies were similarly studied. Electron micros...
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Electron microscopy is arguably the most powerful tool for spatial imaging of structures. As such, 2D and 3D microscopies provide static structures with subnanometer and increasingly with angstrom-scale spatial resolution. Here we report the development of 4D ultrafast electron microscopy, whose capability imparts another dimension to imaging in general and to dynamics in particular. We demonst...
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Heightened interest in the cytology of certain free-living flatworms has been evident in the literature throughout the past year (5, 6, 10-12). The variety of problems dealt with by various investigators reflects the abundance of curious cellular specialization found in this group of animals. Moreover, the suitability of the Turbellarians for the study of regenerative processes is well recogniz...
متن کاملExperiments on fixation for electron microscopy
The effect of fixation with a simple solution of osmium tetroxide in distilled water was studied by electron microscopy. Exocrine cells of the pancreas and cells of the proximal tubule of the kidney of the mouse were used as test-objects. Partially prepolymerized methacrylate was used for embedding. There did not appear to be any marked disorganization of the cell inclusions. The appearance of ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1986
ISSN: 0022-1554,1551-5044
DOI: 10.1177/34.3.3950387